TABLE 1.
Bacterial strains used in this study
| Strain | Relevant characteristics | Source (reference) |
|---|---|---|
| E. coli | ||
| NEB5α | fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | New England BioLabs |
| BL21(DE3) | fhuA2 [lon] ompT gal (λ DE3) [dcm] ΔhsdS λ DE3 = λ sBamHIo ΔEcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 Δnin5 | New England BioLabs |
| TNE012 | ompA ompB tsx | (50) |
| P. aeruginosa | ||
| PAO1 | Clinical isolate | Manoil lab Washington mutant library |
| YHP17 | Clinical isolate | This study |
| PAO6609 | met-9011 amiE200 strA pvd-9 | (26) |
| K1407 | PAO6609 tonB1 | (23, 51) |
| K1408 | PAO6609 tonB2 | (23) |
| MS231 | PAO6609 tonB3 | (52) |
| MS233 | PAO6609 tonB2 tonB3 | (52) |
| PW8161 | PAO1 fptA | Manoil lab Washington mutant library |
| PAO1 Δrmd | CPA deficient | (53) |