FIG 6.

RipA depletion sensitizes M. tuberculosis to multiple cell wall-targeting drugs and to the frontline TB drug rifampin. (A) Violin plots of cell lengths upon ripA silencing with the addition of anhydrotetracycline (atc). Significance was determined by a Kruskal-Wallis test; ****, P < 0.0001; ns, not significant. (B) Quantification of the proportion of cells with at least one septum. The bar height represents the percentage of cells that contain 1 or more septa. The colored segments within the bars represent the proportions of cells with different numbers of septa. (C) MIC profiles of wild-type and ripA-tetOFF strains. Anhydrotetracycline (500 ng/ml) was added to cultures at the time of drug exposure. Growth was measured 14 days after addition of drug and atc. Data are representative of two independent experiments.