Figure 2.

Internalization studies with peptide 2. A) For flow cytometry peptide 2 (30 μm) was incubated with cells for 0 min (blue), 10 min (orange), and 30 min (green). No peptide was added in the negative control (red). Analysis revealed an uptake of peptide 2 only by M21 cells. B) Live cell imaging of peptide 2 confirmed specific uptake only for M21 cells. For a better signal‐to‐noise ratio higher concentrations of peptide 2 (10 μm) were applied. The gain was adjusted in channel 1 to ensure the absence of peptide 2 in M21L cells. White spots in merged channels represent colocalization with lysosome.