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. 2019 Dec 16;20(3):726–738. doi: 10.1111/ajt.15707

Figure 5.

Figure 5

The proteasome activity within apoptotic exosome‐like vesicles (ApoExo) is required for induction of tertiary lymphoid structure (TLS) formation and for anti‐perlecan/LG3 production. Allografted mice were injected with ApoExo that were generated from serum‐starved murine epithelial cells (mECs) treated with bortezomib (n = 11) or the vehicle (n = 10) for 3 weeks posttransplantation: (A) Aortic allograft sections stained with H&E, CD20, CD3, AID, and IL‐17 (magnification: 5×, magnification of right inset panels: 20×). (B) Ratio intima/media ratio in the allografts. (C) Mean number of TLS per allograft. Neointima‐media and perivascular quantification of CD20+ B cells (D), CD3+ T cells (E), AID (F), and IL‐17 (G) staining in each high‐power field of the murine allografts. Anti‐LG3 (H) and ANA (I) IgG levels in sera from wild‐type allografted mice after 3 weeks of intravenous injections with ApoExo generated from serum‐starved mECs treated with bortezomib or the vehicle. Data were pooled from three independent experiments and expressed as means ± SEM. Comparison with the vehicle was done using a Student's t test