Figure 6.

The proteasome activity within apoptotic exosome‐like vesicles (ApoExo) is required for the recruitment of RORγ⁺IL‐17⁺γδT cells to the allograft. Allografted mice were injected with ApoExo that were generated from serum‐starved murine epothelial cels treated with bortezomib (n = 10) or the vehicle (n = 9) for 3 weeks posttransplantation: CD3⁺TCRγ⁺ T cell aortic allograft infiltrates were analyzed by flow cytometry for IL‐17 and RORγ expression. (A) Number of allograft infiltrating of CD3⁺ expressing TCRβ or γ for each condition. (B) Density plot of CD3TCRβ⁺ and CD3⁺TCRγ⁺ cells expression of RORγ and IL‐17. (C) Percentage of CD3⁺TCRγ⁺ infiltrate expressing RORγ and or IL‐17 for each condition. Data were pooled from three independent experiments and expressed as means ± SEM