Figure 2.

Elevated levels of CCL25 and CXCL10 in salivary glands from pSS patients correlate and in vitro these cytokines induce synergistic chemotaxis of Th cells. A, B, mRNA expression of the following chemokines and their receptors were evaluated in labial salivary gland biopsies (tissue lysates) from primary Sjögren's syndrome (pSS, black triangles) and non‐Sjögren's sicca (non‐SS, indicated as nSS) patients: CXCL10 and CXCR3, CCL17 and CCR4, CCL20 and CCR6 and CCL27 and CCR10, showing elevated expression of CXCL10 and CCR6 and trends towards increases in CXCR3, CCL20 and a decrease of CCL27. Boxplots with medians with interquartile range are shown, non‐SS: n = 9, pSS: n = 9, (C) expression of CXCL10 strongly correlated with CCL25 expression and (D) in a transwell chemotaxis assay, synergistic migration of Th cells is induced upon a combination of CCL25 and CXCL10 (n = 8). Mean with SEM are shown, *P < .05