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. 2020 Feb 6;87(3):339–346. doi: 10.1002/ana.25676

Figure 1.

Figure 1

Scn8a antisense oligonucleotide (ASO) treatment reduces the abundance of Scn8a transcript in wild‐type neurons and brain. (A) Cartoon of the 3′ end of the Scn8a gene. The last 2 exons are represented by boxes separated by a line representing the last intron. Coding sequences within the exons are shaded. The position of the stop codon that terminates translation is marked as “stop.” The sequence of the 20 base pair (bp) Scn8a ASO is shown. The ASO sequence is identical to the genomic sequence of the 3′ untranslated region (UTR) at a position approximately 500 bp downstream from the translation stop codon within the last exon of the gene. (B) Primary cortical neurons from E14 embryos of strain C57BL/6J were cultured for 3 days with the indicated concentration of Scn8a ASO. (C) The indicated dose of ASO was administered to C57BL/6J mice on postnatal day 2, and the abundance of Scn8a transcript in brain RNA was measured at postnatal day 21. Five or 6 animals were treated with each dose; each symbol represents data from 1 animal.