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Graphical overview of the experimental design. In short, freshly isolated rat cardiomyocytes were kept in culture for up to 50 hours to allow for the 4mtD3cpv Cameleon (MitoCam) to be expressed. Mitochondrial and cytosolic (in untransfected cells by Fura‐4) calcium concentration was measured in electrically stimulated cardiomyocytes. Pictures are from Servier Medical Art
