Figure 2.

Functionality of the CN–NFAT pathway in primary human monocytes. A) Calcium flux after ionomycin or curdlan (dectin‐1 ligand) treatment of primary human monocytes. B and C) Fluorescence microscopy images of primary human monocytes showing NFAT1 translocation from the cytoplasm to the nucleus 45 min after treatment with Pam3CSK4 and zymosan or with ionomycine and zymosan in the presence or absence of cyclosporine A. The inhibitor was administered 60 min prior to the trigger. Quantification of nuclear translocation was performed using ImageJ software and a nuclear/cytoplasmic ratio plug‐in