Figure 3.

Regulation of PIF4 mRNA and protein levels in thermomorphogenesis in Arabidopsis. (a) Relative expression of PIF4 in 5‐d‐old Col‐0 and (b) in Col‐0 and pif7‐1 mutants. Seedlings were grown as in Fig. 2(a). Gene expression values were calculated as fold induction relative to a Col‐0 sample at 21°C, t = 0 (a) and t = 90 min (b). n = 3 (biological) with three technical replicas for each RNA sample. Data are means, ± 2 SE. Different letters indicate significant differences (P < 0.05). (c) PIF4 protein levels in the indicated genotypes detected with anti‐PIF4 antibody from total protein extracts after 3 h of 21°C and 28°C treatment in 5‐d‐old long day‐grown seedlings treated at ZT2. DET3 was used as a loading control.