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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Exp Gerontol. 2020 Feb 20;133:110885. doi: 10.1016/j.exger.2020.110885

Figure 5: PA supplementation had no effect on osteoblastic mineralization.

Figure 5:

BMSCs isolated from C57BL/6 humerii were induced to differentiate using osteogenic medium, and bone mineralization was determined by alizarin red staining as described in Methods. Shown in the left panel are representative wells, and their quantification using Photoshop is illustrated in the right panel. BMSC mineralization was significantly lower in the mice that received the low-protein diet, but PA had no additional effect. Results are representative of at least three separate experiments