Figure 4.

CPL‐loaded membrane supports cell proliferation in CFU‐F assay. (A) Representative CFU‐F dishes of BM‐MSCs grown in either standard expansion media (top dish) or serum‐free DMEM with the additional CPL‐loaded membrane as a source of released growth factors and cytokines (bottom dish). (B) Representative individual colonies of cells grown in expansion media (top) or CPL‐loaded membrane and DMEM (bottom). Three BMA donors were tested on a single CPL product. Images were collected using a photo scanner at 1200 dpi. (C) Comparison of average colony area, density, and total number between cells treated with a CPL‐loaded scaffold and expansion media (EM). An unpaired t‐test found no significant difference between CPL of EM.