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A) Principle of the Ehrlich's assay. B) Kinetic characterization of HapB (error bars show the standard deviation of three separate measurements). C) Flexibility of HapB for substrates 5 a–k as determined by Ehrlich's assay and normalised relative to the A 555 value obtained with 5 a: 5 a (red) natural substrate H2MAP; 5 b–g (green) analogues with modifications at C3, 5 h–k (blue) analogues with modifications at C2.
