Figure 2.

Schematic representation of an organoid or organ‐on‐chip model. Inlet: In a controlled way, special nutrients and morphogens can flow through the chambers. Diffusion of molecules from the two lateral chambers to the central chamber, leading to binding of these molecules to the extracellular matrix and membrane receptors, is facilitated by the semipermeable membrane between the two lateral chambers and the central chamber. Using this model, gradients of different ligands and/or morphogens can be established in the central chamber. The central chamber can be filled with (injectable) extracellular matrix containing organoids or mini‐organs. Outlet: Medium containing CO2 and metabolic waste products can be removed from the culture via the outlets.