Figure 2.

A) Control reactions using NDO H295A under light (○) and dark (•) conditions with (+) and without (−) 100 μm EY, RB, or CE in the presence of NDO H295A (red bars) or with an empty vector control (gray bars) in 50 mm MOPS. Values for the empty vector control were the highest that have been achieved when the maximum light intensity of 112 μE L⁻¹ s⁻¹ was applied. B) Effect of photosensitizer concentration on product yield. Different concentrations of CE used in combination with MES as electron donor in the light‐driven whole‐cell hydroxylation reaction employing NDO H295A. Reaction conditions: 0–320 μm photosensitizer, 10 mm 3, 50 mm MES, 100 g_WCW L⁻¹ whole cells (E. coli JM109 (DE3)_pDTG141_NDO H295A, 19 h expression), 50 mm MES, white light (max. 112 μE L⁻¹ s⁻¹), 30 °C, 140 rpm.