Table 2.
The combination of photosensitizer and electron donor is a crucial factor for the efficiency of the light‐driven reaction.
|
Enzyme |
Photo‐ sensitizer/ |
|
Products |
|
Whole‐cell activity |
|
Apparent quantum yield |
|||
|---|---|---|---|---|---|---|---|---|---|---|
|
|
Electron donor |
|
Max. conc. [mm][a] |
Diast. ratio cis/trans‐3 b [%][b] |
Distrib. 3 a/3 b [%][c] |
|
Specific activity[d] [mU gWCW −1] |
Productivity [mm h−1] |
|
[%] |
|
CDO M232A |
EY/EDTA |
|
3.7±0.4 |
100:0 |
3:97 |
|
29 |
0.18 |
|
0.09 |
|
EY/MOPS |
|
6.8±0.03 |
100:0 |
0:100 |
|
21 |
0.13 |
|
0.06 |
|
|
EY/MES |
|
4.7±0.2 |
100:0 |
4:96 |
|
41 |
0.25 |
|
0.12 |
|
|
RB/EDTA |
|
6.8±0.3 |
96:4 |
4:96 |
|
265 |
1.59 |
|
0.78 |
|
|
RB/MOPS |
|
7.3±0.6 |
95:5 |
4:96 |
|
156 |
0.94 |
|
0.46 |
|
|
RB/MES |
|
5.8±0.4 |
95:5 |
3:97 |
|
275 |
1.65 |
|
0.81 |
|
|
CE/EDTA |
|
4.0±0.01 |
94:6 |
2:98 |
|
43 |
0.26 |
|
0.13 |
|
|
CE/MOPS |
|
8.6±0.6 |
88:12 |
0:100 |
|
102 |
0.61 |
|
0.30 |
|
|
|
CE/MES |
|
8.3±0.08 |
90:10 |
0:100 |
|
124 |
0.75 |
|
0.37 |
|
NDO H295A |
EY/EDTA |
|
4.7±0.2 |
96:4 |
4:96 |
|
47 |
0.23 |
|
0.11 |
|
EY/MOPS |
|
7.7±0.4 |
83:17 |
2:98 |
|
61 |
0.37 |
|
0.18 |
|
|
EY/MES |
|
7.4±0.3 |
86:14 |
4:96 |
|
87 |
0.52 |
|
0.26 |
|
|
RB/EDTA |
|
7.5±1.0 |
97:3 |
0:100 |
|
53 |
0.32 |
|
0.16 |
|
|
RB/MOPS |
|
7.5±1.2 |
95:5 |
2:98 |
|
148 |
0.89 |
|
0.44 |
|
|
RB/MES |
|
7.9±0.6 |
97:3 |
3:97 |
|
79 |
0.45 |
|
0.22 |
|
|
CE/EDTA |
|
5.5±0.5 |
94:6 |
6:94 |
|
50 |
0.30 |
|
0.15 |
|
|
CE/MOPS |
|
7.3±0.4 |
95:5 |
18:82 |
|
143 |
0.86 |
|
0.42 |
|
|
CE/MES |
|
8.5±0.4 |
86:14 |
0:100 |
|
107 |
0.64 |
|
0.32 |
|
Reaction conditions: [3]=10 mm; [whole cells]=100 gWCW L−1 (E. coli JM109 (DE3)_pDTG141_NDO H295A or E. coli JM109_pCDOv1_CDO M232A); sodium phosphate buffer (pH 7.2, 50 mm) when using 25 mm EDTA, otherwise 50 mm MES/MOPS buffer; white‐light illumination (max. 112 μE L−1 s−1).[a] Sum of 3 a and 3 b; time points for determination were chosen at maximum product concentration during the time course of the reaction. [b] The diastereomeric ratio was determined after 4–6 hours of reaction.[c] Determined after 24 hours.[d] Determined from the liner range of product formation determined from the kinetic profiles for each reaction (Figures S19–S24).