Figure 3.

Biochemical analysis of chondrocytes in three‐dimensional (3D) constructs. (A) The 3D constructs were cultured with cyclic or permanent exposure. (B) After 4 weeks in culture with insulin‐like growth factor‐1 (IGF1), IGF2, or sprifermin or (C) with bone metamorphic protein 7 (BMP7), C‐type natriuretic peptide (CNP), or sprifermin the glycosaminoglycan (GAG) and HPro content were analyzed. Data represent the mean ± standard error of the mean (SEM). *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001 versus control (culture medium only).