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. Author manuscript; available in PMC: 2020 Jul 8.
Published in final edited form as: Sci Transl Med. 2020 Jan 8;12(525):eaay7205. doi: 10.1126/scitranslmed.aay7205

Fig. 5. SAHA induces switch in skeletal muscle fiber composition and improves mitochondrial function.

Fig. 5.

In vivo measurements of (A) oxygen consumption. (B) mRNA expression of genes related to mitochondrial biogenesis (Pgc-1α, Pgc-1β, Tfam), glucose metabolism (Glut4), and TCA cycle (Idh3α) in skeletal muscle. (C) Skeletal muscle fiber composition. Assessment of (D) mitochondrial membrane potential, (E) mitochondrial structure, and (F) mitochondrial calcium uptake before and after histamine administration in HeLa cells. Scale bars=3 μm. (G) Oxygen consumption rate and extracellular acidification rate were measured using Seahorse Technology: basal (1) and maximal (3) mitochondria respiration, uncoupling (4), and proton leak (2) in HeLa cells. Data shown are means ± SEM. Statistical significance was determined by (A-C) Kruskal-Wallis tests with multiple comparisons using two-stage linear step-up procedure and (D, F, G) analysis of variance (ANOVA) followed by Bonferroni post hoc testing to compare mean values between groups. §P < 0.05 between b+SAHA and sham. #P < 0.05, ##P < 0.01 between b+SAHA and b+veh. The number of animals included in each parameter is reported in table S7.