TABLE 2.

SNPs identified to have genome-wide suggestive effects on serum biochemical traits in Holstein cattle using a mixed-effect linear model.

Trait1	SNP-name	Chr	Position	Model2	P-value	Nearest gene	Distance3
NEFA	BovineHD2300011114	23	38421269	MLM	9.63E−06	–	–
AST	Hapmap24000-BTA-150203	11	71811673	MLM	2.01E−05	BRE	Within
TCHO	ARS-BFGL-NGS-65263	8	1.07E + 08	MLM	1.16E−05	PAPPA	Within
CHE	BovineHD0100005950*	1	20036999	MLM	1.85E−05	–	–
	BovineHD0200000997	2	3780881	MLM	6.33E−06	–	–
	ARS-BFGL-NGS-115158*	29	21828399	MLM	1.30E−05	–	–
DBIL	BovineHD1500021175*	15	73378270	MLM	1.73E−05	–	–
CR	ARS-BFGL-NGS-1888	23	20839913	MLM	1.74E−05	OPN5	D 5595
CK	chr17 71438606	17	71438606	MLM	1.39E−05	LOC104974701	Within
BHB	ARS-BFGL-NGS-113393	6	14179168	MLM	1.31E−05	ZGRF1	Within
SUN	BovineHD2900002496	29	8779426	MLM	1.21E−05	PRSS23	U 9262
LDL	BovineHD0400034698	4	1.18E + 08	MLM	1.39E−05	–	–
	BovineHD0800028900*	8	97883896	MLM	6.29E−06	LOC101903458	D 72327
	BTB-00442438*	10	89826995	MLM	2.21E−05	SPTLC2	Within
VLDL	ARS-BFGL-NGS-114594	5	22599252	MLM	1.76E−05	LOC107132468	U 31230

¹NEFA, non-esterified fatty acid; AST, aspartate aminotransferase; TCHO, total cholesterol; CHE, cholinesterase; DBIL, direct bilirubin; CR, serum creatinine; CK, creatine kinase; BHB, β-hydroxybutyric acid; SUN, serum urea nitrogen; LDL, low-density lipoprotein; VLDL, very low-density lipoprotein. ²MLM, mixed-effect linear model. ³The distance from the SNP locus to the gene (unit: bp); U indicates that the SNP site is located in the downstream of the gene; D indicates that the SNP site is located in the downstream of the gene; Within indicates that the SNP locus is located within the gene. *SNPs identified to have genome-wide significant effects on serum biochemical traits in Chinese Holstein cattle using FLM (fixed linear model).