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. Author manuscript; available in PMC: 2020 Oct 3.
Published in final edited form as: Mol Cell. 2019 Aug 29;76(1):82–95.e7. doi: 10.1016/j.molcel.2019.07.017

Figure 3. Loss of SUGP1 Recapitulates the Splicing Defects of K700E SF3B1.

Figure 3.

(A) HEK293T cells were transfected with a negative control siRNA (siC), or one of two independent siRNAs targeting SUGP1, followed by western blotting.

(B) RT-PCR products of the cryptic 3′ss (open arrowheads) and canonical 3′ss (solid arrowheads) of the indicated genes in HEK293T cells as in (A).

(C) Each of the indicated WT (shown in green) and mutant (shown in red) minigenes was cotransfected either with expression plasmid for HA-tagged WT (W) or K700E (K) SF3B1 (left panels), or with one of two independent siRNAs targeting SUGP1 (right panels) to HEK293T cells, followed by RT-PCR of the cryptic 3′ss (open arrowheads) and canonical 3′ss (solid arrowheads) from the minigenes. As indicated, an empty vector plasmid (Vec) and a negative control siRNA (siC) were also used.

See also Figures S2 and S3.