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AMA (a–c) and liver biochemical markers (d–f) along time in PBC patients and BN/AMA + subjects. BN vs time: temporal change in BN group. BN/time vs PBC/time comparison of temporal change in BN/AMA+ subjects versus PBC patients, BN/AMA+ biochemically normal AMA-positive individuals (BLUE), PBC primary biliary cholangitis (RED). Index anti-PDC-E2 IgG: arbitrary units (AU) calculated relative to cut-off
