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. 2020 Mar 10;8:32. doi: 10.1186/s40168-020-00802-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Transplantation of IM leads to similar symptom of WFS. a Schematic representation of IMT procedures. Control shrimp (n = 30) were received IMT from different donors to evaluate the causative role of IM dysbiosis to WFS. The shrimp suffered from WFS after IMT were selected as WFS donors for the subsequent IMT. To determine whether WFS shrimp can regress by IMT from donors without WFS, WFS shrimp (n = 22) were received IMT from Control donors and PBS. b Incidence of recipient shrimp suffered from WFS after the first IMT and the subsequent IMT. c Histological pathology of WFS shrimp. The black arrows point towards the disease signs of WFS (dropped epithelial cell, reduced, or disappeared microvilli, thinner mid intestine). d Observation of the progressive severity of WFS after IMT. e The WFS shrimp recovered to health by IMT from control donors. f Histological pathology confirmed that the diseased shrimp regressed to health