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. 2020 Mar 10;17:33. doi: 10.1186/s12985-020-01299-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Identification of ChiVMV infecting tobacco plants. a Typical symptoms of chlorosis and round bright spots on the leaves of field-grown tobacco plants collected from Luzhou city, Sichuan province, China. b Viral detection in selected field-grown tobacco leaves using four universal primers by RT-PCR. lane M, Trans2K DNA marker; lane 1, primers PotyF/PotyR; lane 2, primers CMVCPf/CMVCPr; lane 3, primers Tob-Uni1/Tob-Uni2; lane 4, primers TSWVf/TSWVr