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. 2020 Mar 11;51:40. doi: 10.1186/s13567-020-00764-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Gross lesions, histopathology and apoptotic cells found in chickens infected withG. anatisΔgtxAmutant orG. anatiswild-type (WT) strain. Ovaries from 29-week old laying hens. Two days post-inoculation with either G. anatis ΔgtxA or G. anatis WT. AG. anatis ΔgtxA infection group. Oophoritis with vascular engorgement and slight edema. BG. anatis wild-type. Diffuse purulent oophoritis and folliculitis with ruptures follicles. C Hematoxylin and eosin (HE)-straining of the follicle in ovarian tissue from the G. anatis ΔgtxA infection group. Focal oophoritis with the presence of inflammatory cells in the stroma and vascular engorgement. Infiltrates of few heterophilic granulocytes and mononuclear cells. D HE-straining of the ovary of the G. anatis WT infection group. Purulent oophoritis with heavy infiltration of inflammatory cells and granuloma formation (black arrows) in the follicle.