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. 2020 Mar 11;51:40. doi: 10.1186/s13567-020-00764-2

Table 2.

List of the primers used in RT-qPCR analysis of mRNA expression of selected host genes

Name Primer Primer sequence(5′–3′) Accession Conc. (mM)
bcl-2 Forward GATGACCGAGTACCTGAACC NM205339 0.2
Reverse CAGGAGAAATCGAACAAAGGC
bax Forward TCCTCATCGCCATGCTCAT XM422067 0.4
Reverse CCTTGGTCTGGAAGCAGAAGA
caspase-8 Forward TGGCCCTCTTGAACTGAAAG AY057940 0.4
Reverse TCCACTGTCTGCTTCAATACC
caspase-9 Forward CGAAGGAGCAAGCACGACAG AY057940 0.2
Reverse CCGCAGCCCTCATCTAGCAT
caspase-3 Forward TGGCCCTCTTGAACTGAAAG AY057940 0.4
Reverse TCCACTGTCTGCTTCAATACC
IL-6 Forward GCTCGCCGGCTTCGA AJ250838 0.2
Reverse GGTAGGTCTGAAAGGCGAACAG
IL-4 Forward AACATGCGTCAGCTCCTGAAT AJ621735 0.4
Reverse TCTGCTAGGAACTTCTCCATTGAA
IL-10 Forward CATGCTGCTGGGCCTGAA J621614 0.4
Reverse CGTCTCCTTGATCTGCTTGATG
TNF-α Forward GCCCTTCCTGTAACCAGATG NM_204267 0.2
Reverse ACACGACAGCCAAGTCAACG
β-actin Forward CCGCTCTATGAAGGCTACGC L08165 0.4
Reverse CTCTCGGCTGTGGTGGTGAA