Fig. 1.

Dose response of the inhibitory effect of rupatadine on IL-6 release from HMC-1 cells. HMC-1 cells were preincubated with rupatadine (Rup) for 10 min at 1, 5, 10, 25 and 50 µM and stimulated with IL-1 (100 ng/ml, for 6 h). Control mast cells were treated with culture medium only for 6 h. After the incubation period, the supernatant solution was collected and IL-6 was assayed by ELISA. Results were analyzed using the unpaired equal, 2-tailed, Student's t test (n = 11). Spontaneous release was below the level of detection. * p < 0.05, significance of comparisons between conditions and samples treated with the trigger alone, as shown by the corresponding brackets.