Fig. 2.

Time course of the inhibitory effect of rupatadine on IL-6 release from HMC-1 cells. HMC-1 cells were preincubated with rupatadine (RUP) at 10 and 25 µM for 10 min, 1, 6, 12 and 24 h and stimulated with IL-1 (at 100 ng/ml, for 6 h). Control mast cells were treated with culture medium only for 6 h. After the incubation period, the supernatant solution was collected and IL-6 was assayed by ELISA. Results were analyzed using the paired equal, 2-tailed, Student's t test (n = 3). * p < 0.05, significance of comparisons between the results of the same concentration at different times of preincubation and 10 min of preincubation, as shown by the corresponding brackets.