Fig. 3.

Dose response of the inhibitory effect of rupatadine on IL-8 release from LAD2 cells. LAD2 cells were preincubated with rupatadine (Rup) at 10 (n = 4), 25 (n = 15) and 50 µM (n = 9) for 10 min and were subsequently stimulated with SP (10 µM) for 24 h. Spontaneous release was below the level of detection. Results were analyzed using the unpaired equal, 2-tailed, Student's t test (for the comparison between SP and 10 or 25 µM rupatadine + SP, for each time of preincubation) and the unpaired unequal, 2-tailed, Student's t test for the rest. * p < 0.05, significance of comparisons between conditions and samples treated with the trigger alone, as shown by the corresponding brackets.