Fig. 6.

Dose response of the inhibitory effect of rupatadine on histamine release from LAD2 cells. LAD2 cells were preincubated with rupatadine (Rup) at 5, 10 and 50 µM for 10 min and stimulated with SP (at 10 µM, n = 9) for 20 min. Histamine in the supernatant and pellet was measured fluorometrically. Spontaneous release was below the level of detection. Results are presented as percent net release, and data were analyzed by the unpaired equal, 2-tailed, Student's t test. * p < 0.05, significance of comparisons between conditions and samples treated with the trigger alone, as shown by the corresponding brackets.