Fig. 1.

Smarcad1 localization in the intestinal tract and Vil-cre-mediated KO. a–e Intestinal epithelium localization of Smarcad1 (red) by IF staining and nuclear counterstaining with DAPI (cyan) shown as maximum intensity projections. Scale bars, 40 μm. a Wild type (WT) small intestinal (SI) epithelium. b WT colon epithelium. c, dVil-cre-mediated tissue-specific knockout of Smarcad1 (KO) in SI (c) and colon (d). e No primary antibody control on WT SI. f, g Representative Western blots of Smarcad1 protein levels in the intestinal tract and H3 loading normalization in WT (f) and KO (g) samples. Villus and crypt SI samples are shown as anterior, central, and posterior fractions of the SI. Protein size markers [kDa] indicated on the left. Quantitation of Western blots (n = 3) is shown in Additional file 1: Fig. S1b