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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: DNA Repair (Amst). 2020 Jan 9;87:102773. doi: 10.1016/j.dnarep.2019.102773

Figure 1. Oxidative Trapping of AP Lyases in DPC in vivo.

Figure 1.

MDA-MB-231 cells were treated with increasing concentrations of CuOP for 1 h (under conditions described in section 2.1), genomic DNA (gDNA) immediately isolated, and 1-μg aliquots assayed for DPC using the appropriate antisera. A, Ku antigen; B, PARP1; C, Polλ. For panel D, WT or POLL−/− MEF were treated and assayed for Ku-DPC. For each set of experiments, a representative blot is shown, with quantification for n= 3 independent experiments, normalized to untreated cells. Error bars show +/− S.D. * indicates p<0.05 (two-tailed Student’s t-test) compared to untreated cells.