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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: DNA Repair (Amst). 2020 Jan 9;87:102773. doi: 10.1016/j.dnarep.2019.102773

Figure 5. Processing of oxidative AP Lyase-DPC.

Figure 5.

MDA-MB-231 cells were treated with 10 μM CuOP for 1 h (under conditions described in section 2.1), then transferred to fresh medium. gDNA was isolated either immediately after the treatment, or at the indicated times of the subsequent incubation. Samples (1μg) were assayed for: A, Ku antigen; B, PARP1; C, Polλ; and D, OGG. Quantification for each set of experiments is shown for each blot. Representative blots are shown, with quantification for n= 3 independent experiments, normalized to untreated cells. Error bars show +/−S.D. for n= 3 independent experiments. * indicates p<0.05 (two-tailed Student’s t-test) compared to the ‘0 min’ time point (defined as 100%).