Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 4;8:132. doi: 10.3389/fbioe.2020.00132

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Bloise, Massironi, Della Pina, Alongi, Siciliani, Manfredi, Biggiogera, Rossi, Ferruti, Ranucci and Visai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Cytotoxicity and internalization of Trastuzumab-conjugated gold nanoparticles differently sized. (A) Dose- and time-dependence cell viability of different cell lines cultured with free Trastuzumab, 2.5Au@PT and 5@AuPT. SKBR-3 (e,f) and MCF-7 cells (c,d) were incubated with 2.5Au@PT or 5Au@PT suspensions with concentration in the range of 0–2 μg/mL. Similarly, the experiment was performed in NIH-3T3 fibroblasts cell line (a,b) as negative control. After 1 and 3 days of incubation, cell viability was expressed as percentage of viability determined in the absence of nanoparticles (set at 100%). (B) Uptake efficiency of 2.5Au@PT and 5Au@PT (2 μg/mL) after 1 and 3 days of incubation were determined by ICP-MS in all the indicated cell lines (a, NIH-3T3; b, MCF-7; c, SKBR-3) and calculated as described in Experimental Section (mean values ± standard deviations; **p < 0.01 and ***p < 0.001).