Skip to main content
. 2020 Mar 4;8:132. doi: 10.3389/fbioe.2020.00132

FIGURE 5.

FIGURE 5

(A) Cell morphology assessed by scanning electron microscopy in ctrl (a), with Trastuzumab (b), 2.5Au@PT (c) and 5Au@PT (d), respectively, after 3 days of incubation. Scale bars = 100 μm and mag: 1000×. After 3 days of culture, all experimental groups [ctr (e), T free (f), 2.5Au@PT (g), and 5Au@PT (h)] were treated with fluorescein diacetate (green cells alive) and propidium iodide (red cells dead) 20× magnification, the scale bar represents 50 μm. (B) Western blotting analysis of survival (a,b), proliferative (a,c), pro (d,e), and anti-apoptotic (d,f) proteins extracted from SKBR-3 after 24 h incubation with the indicated treatments. The activation level of AKT or ERK is presented as a ratio between the phosphorylated and total AKT or ERK protein after normalization to β-actin housekeeping protein signal. Bar graphs show BAX and BCL-XL expression level obtained normalizing β-actin housekeeping protein signal. Statistical significance values are indicated as ***p < 0.001, **p < 0.01, and *p < 0.05.