TABLE 2.

Genetic diversity index of the used EST-SSR markers in Opisthopappus.

Primer	SSRs	Forward primer	Reverse primer	Tm (°C)	T	TP	PPB (%)	He	Ho	PIC
1	ACA(6)	GTTCCACCACCATTG	TGCCTAAGAGAACAGATA	56.15	31	31	49.07	0.147	0.154	0.3667
	CAA(6)	AGTTTGTGAG	ATGAGAT
2	ACA(6)	GTTCCACCACCATTG	GATGGTCAATCAAAGAGT	56.8	34	34	54.95	0.136	0.143	0.2878
	CAA(6)	AGTTTGTGAG	GTGGCTA
3	ACA(6)	GTTCCACCACCATTG	AGGAATGGGAATACAAA	56.85	27	27	49.12	0.131	0.137	0.3372
	CAA(6)	AGTTTGTGAG	GACGAAAA
4	AAAC(5)	CGGAGAAATAGTAAGTCG	GAGTAGGCATGAGTGGGT	49.7	52	52	62.65	0.183	0.192	0.3860
5	AAAC(5)	ACTCCTACAGTGTCGGTG	AATACTACTGCTACTGGCTCTT	53.65	39	39	66.94	0.191	0.200	0.4411
6	AGCA(5)	GAGTGGTGTGGTGTTTTACTGTCGT	ACGATTTGTGCTGTTCTGGGAGTGG	57.35	31	31	46.01	0.116	0.122	0.3411
7	GAA(6)	CAAGGTCGTCAACTCATAA	CTGCTGTGATGGGAAAGA	51.3	44	44	61.96	0.171	0.180	0.3863
8	GAA(6)	ATGTTCCGTTTGACGAGA	GCTACTCCTGACCGACTT	53.85	47	47	48.82	0.138	0.145	0.3756
9	AGCA(5)	AACAATAAGTCCGCCACA	ACTCACCCAAGAATACATCG	53.45	38	38	49.31	0.166	0.174	0.3958
10	AGCA(5)	ATGTGCTTTACCCTTACC	GGAGAAGAGTAAAGAGGAGGCT	53.75	40	40	54.47	0.171	0.180	0.3817
11	AGCA(5)	GATTTGTGCTGTTCTGGGAG	GAGGCTGGGATTTGGGTT	55.7	29	29	58.26	0.136	0.143	0.2919

T, total number of amplified bands; TP, total number of polymorphic bands; PPB, percentage of polymorphism; He, expected heterozygosity; Ho, observed heterozygosity; PIC, polymorphic information content.