Table 2.
Observed Response in Box–Behnken Design for Optimization of DH Loaded TENVs
| Formulation | Independent Variables | Dependent Variables | PDI | ||||||
|---|---|---|---|---|---|---|---|---|---|
| A: PC Concentration (%w/w) | B: Ethanol Concentration (%w/w) | C: SDC Concentration (% w/w) | Y1: EE% | Y2: Vesicle Size (nm) | Y3: Zeta Potential (mV) | Y4: Q8h (%) | Y5: Q24 (µg/cm2) | ||
| F1 | 1 | 10 | 0.15 | 39.80± 1.75 | 168.50± 11.03 | -19.20± 3.20 | 76.70± 13.57 | 238.40± 6.96 | 0.32 |
| F2 | 2 | 25 | 0.15 | 54.60± 4.13 | 195.10± 19.69 | -32.20± 5.56 | 67.10± 10.00 | 334.70± 24.10 | 0.47 |
| F3 | 3 | 25 | 0.1 | 75.50± 3.72 | 285.20± 24.01 | -42.30± 6.25 | 53.10± 3.50 | 345.83± 21.79 | 0.27 |
| F4 | 2 | 40 | 0.1 | 61.50± 4.61 | 220.40± 20.05 | -36.40± 4.30 | 63.20± 12.25 | 269.50± 25.00 | 0.50 |
| F5 | 2 | 25 | 0.15 | 55.70± 3.39 | 199.20± 13.26 | -32.10± 3.60 | 67.70± 4.55 | 334.23± 23.91 | 0.24 |
| F6 | 1 | 40 | 0.15 | 35.50± 2.06 | 165.40± 20.44 | -26.90± 4.80 | 81.30± 11.85 | 259.13± 27.01 | 0.42 |
| F7 | 1 | 25 | 0.1 | 42.40± 2.79 | 179.60± 18.96 | -24.60±5.36 | 71.40± 7.17 | 174.38± 15.12 | 0.39 |
| F8 | 3 | 10 | 0.15 | 69.50± 3.80 | 273.40± 19.33 | -38.30± 1.00 | 57.40± 4.19 | 409.50± 21.50 | 0.48 |
| F9 | 2 | 25 | 0.15 | 54.20± 1.06 | 193.60± 22.84 | -32.20± 8.42 | 68.60± 3.74 | 334.10± 22.10 | 0.39 |
| F10 | 2 | 10 | 0.2 | 53.10± 2.14 | 190.40± 17.86 | -31.10±8.94 | 73.60± 6.88 | 398.60± 23.01 | 0.33 |
| F11 | 3 | 40 | 0.15 | 66.90± 5.95 | 262.10± 22.59 | -43.80± 9.41 | 60.70± 4.06 | 429.83± 27.01 | 0.39 |
| F12 | 2 | 25 | 0.15 | 53.70± 3.42 | 192.70± 25.29 | -32.10±7.60 | 68.20± 3.23 | 334.87± 20.12 | 0.36 |
| F13 | 2 | 40 | 0.2 | 48.60± 3.59 | 187.90± 8.77 | -37.00±6.95 | 78.90± 9.63 | 418.43± 26.87 | 0.22 |
| F14 | 1 | 25 | 0.2 | 32.90± 0.55 | 142.60± 10.45 | -26.00± 2.76 | 85.90± 14.45 | 322.06± 23.98 | 0.17 |
| F15 | 3 | 25 | 0.2 | 59.70± 2.25 | 218.50± 14.00 | -43.50±7.61 | 65.10± 4.70 | 493.74±30.08 | 0.38 |
| F16 | 2 | 25 | 0.15 | 56.30± 3.36 | 206.10± 9.63 | -33.20± 3.39 | 68.60± 5.00 | 334.53± 19.28 | 0.44 |
| F17 | 2 | 10 | 0.1 | 62.20± 4.14 | 245.90± 23.41 | -27.20± 2.11 | 59.10± 3.62 | 251.28 ±21.72 | 0.32 |
| Fop | 2.68 | 32.66 | 0.2 | ||||||
| Observed values | 52.62 | 216.53 | -38.14 | 61.69 | 467.87 | ||||
| Predicted values | 56.55 | 208.48 | -41.75 | 69.90 | 471.29 | ||||
Note: Data are mean values (n = 3) ± SD.
Abbreviations: PC, phosphatidyl choline; SDC, sodium deoxycholate; EE%, entrapment efficiency percent; Q8h, cumulative release after 8 h (%); Q24, cumulative amount permeated/unit area in 24 h; PDI, polydispersity index; Fop, optimal TENV.