Table 2.
Top 20 Most Abundant Plasma Proteins Adsorbed on Magnetosomes and PEG-Fe3O4 Identified by LC-MS/MS
| Protein IDs | Gene Names | UniProt Names | Protein Names | % PEG-Fe3O4 | % Magnetosomes | pI | GARAY | MW.[kDa] |
|---|---|---|---|---|---|---|---|---|
| P02649 | APOE | APOE_HUMAN | Apolipoprotein E | 4.18 | 9.64 | 5.65 | −0.596 | 36.154 |
| P04004 | VTN | VTNC_HUMAN | Vitronectin | 1.14 | 8.90 | 5.55 | −0.723 | 54.305 |
| P02768-1 | ALB | ALBU_HUMAN | Serum albumin | 0.78 | 8.86 | 5.92 | −0.377 | 69.366 |
| P01834 | IGKC | IGKC_HUMAN | Ig kappa chain C region | 5.01 | 7.95 | 6.075 | −0.537 | 11.765 |
| P02776 | PF4 | PLF4_HUMAN | Platelet factor 4 | 1.03 | 5.23 | 8.93 | 0.351 | 10.845 |
| P10909-4 | CLU | CLUS_HUMAN | Clusterin | 4.81 | 4.43 | 5.88 | −0.665 | 48.803 |
| P18428 | LBP | LBP_HUMAN | Lipopolysaccharide-binding protein | 6.46 | 3.85 | 6.23 | 0.062 | 53.383 |
| P01857 | IGHG1 | IGHG1_HUMAN | Ig gamma-1 chain C region | 1.35 | 3.12 | 7.778 | −0.365 | 43.911 |
| P0DOY3 | IGLC3 | IGLC3_HUMAN | Immunoglobulin lambda constant 3 | 1.60 | 3.03 | 6.91 | −0.439 | 11.265 |
| K7ERI9 | APOC1 | APOC1_HUMAN | Apolipoprotein C-I | 14.05 | 2.96 | 8.01 | −0.119 | 8.647 |
| P12259 | F5 | FA5_HUMAN | Coagulation factor V | 0.39 | 2.65 | 5.68 | −0.596 | 252.23 |
| P01042 | KNG1 | KNG1_HUMAN | Kininogen-1 | 1.55 | 2.60 | 6.34 | −0.757 | 71.957 |
| P02647 | APOA1 | APOA1_HUMAN | Apolipoprotein A-I | 1.11 | 1.75 | 5.56 | −0.717 | 30.777 |
| P02675 | FGB | D6REL8_HUMAN | Fibrinogen beta chain | 2.59 | 1.74 | 8.54 | −0.758 | 55.928 |
| P02679-2 | FGG | C9JC84_HUMAN | Fibrinogen gamma chain | 2.19 | 1.70 | 5.37 | −0.575 | 49.496 |
| Q5T985 | ITIH2 | ITIH2_HUMAN | Inter-alpha-trypsin inhibitor heavy chain H2 | 4.61 | 1.53 | 6.4 | −0.293 | 105.21 |
| C9JV77 | AHSG | C9JV77_HUMAN | Alpha-2-HS-glycoprotein | 2.70 | 1.37 | 5.43 | −0.184 | 39.411 |
| P02671 | FGA | FIBA_HUMAN | Fibrinogen alpha chain | 2.05 | 1.27 | 5.7 | −0.822 | 94.972 |
| P01859 | IGHG2 | IGHG2_HUMAN | Ig gamma-2 chain C region | 0.97 | 1.24 | 7.66 | −0.419 | 35.9 |
| P59666 | DEFA3 | DEF3_HUMAN | Neutrophil defensin 3 | 0.47 | 1.22 | 5.71 | 0.229 | 10.245 |
| P27169 | PON1 | PON1_HUMAN | Serum paraoxonase/arylesterase 1 | 3.49 | 1.11 | 5.08 | −0.091 | 39.731 |
| P02748 | C9 | CO9_HUMAN | Complement component C9 | 2.27 | 0.94 | 5.43 | −0.45 | 63.173 |
| P05546 | SERPIND1 | HEP2_HUMAN | Heparin cofactor 2 | 2.29 | 0.90 | 6.41 | −0.236 | 57.07 |
| P02760 | AMBP | AMBP_HUMAN | Protein AMBP | 1.85 | 0.64 | 5.95 | −0.299 | 38.999 |
| P0C0L5 | C4B | CO4B_HUMAN | Complement C4-B | 1.22 | 0.62 | 6.89 | −0.253 | 192.75 |
| P19827 | ITIH1 | ITIH1_HUMAN | Inter-alpha-trypsin inhibitor heavy chain H1 | 1.46 | 0.43 | 6.31 | −0.282 | 101.39 |
| B0YIW2 | APOC3 | APOC3_HUMAN | Apolipoprotein C-III | 2.14 | 0.21 | 5.23 | −0.086 | 12.815 |
Notes: %Protein relative abundance, calculated by protein’s Av.iBAQ intensity to total Av.iBAQ intensity of identified corona proteins on nanoparticle. Protein names in italic represent the overlapped 13 proteins in the top 20 corona proteins identified on magnetosome and PEG-Fe3O4.