Table 1. Kinetic Parameters of LmFH Enzymes.

enzyme	substrate	Km (mM)	Vmax (μmol min–1 mg–1)	kcat (s–1)	kcat/Km (×103 M–1 s–1)	reaction condition	ref
LmFH-1	S-malate	1.2 ± 0.3	0.50 ± 0.03	0.55 ± 0.03	0.5 ± 0.1	aerobic	(9)
LmFH-1	S-malate	13.0 ± 0.8	338 ± 6	364 ± 6	28 ± 2	anaerobic	this study
LmFH-2	S-malate	5.7 ± 0.2	22.7 ± 0.5	24.9 ± 0.5	4.4 ± 0.2	aerobic	(9)
LmFH-2	S-malate	11.5 ± 0.5	485 ± 6	537 ± 7	47 ± 3	anaerobic	this study
LmFH-2-H334A	S-malate	4.6 ± 0.1	52.0 ± 0.4	57.6 ± 0.4	12.5 ± 0.4	anaerobic	this study
LmFH-2-R421A	S-malate	4.1 ± 0.3	0.130 ± 0.002	0.144 ± 0.002	0.035 ± 0.003	anaerobic	this study
LmFH-2-D135A	S-malate	5.3 ± 0.3	0.068 ± 0.001	0.075 ± 0.001	0.014 ± 0.001	anaerobic	this study
LmFH-2-T467A	S-malate	4.4 ± 0.2	0.047 ± 0.001	0.052 ± 0.001	0.012 ± 0.001	anaerobic	this study
LmFH-2-R471A	S-malate	4.3 ± 0.2	0.034 ± 0.001	0.038 ± 0.001	0.009 ± 0.001	anaerobic	this study
LmFH-2-R173A	S-malate	4.7 ± 0.4	0.030 ± 0.001	0.033 ± 0.001	0.007 ± 0.001	anaerobic	this study
LmFH-1	fumarate	1.3 ± 0.3	1.8 ± 0.3	1.9 ± 0.3	1.5 ± 0.6	aerobic	(9)
LmFH-1	fumarate	4.2 ± 0.6	1,076 ± 50	1,159 ± 54	280 ± 50	anaerobic	this study
LmFH-2	fumarate	1.9 ± 0.3	44 ± 5	49 ± 5	26 ± 7	aerobic	(9)
LmFH-2	fumarate	4.6 ± 0.5	1,355 ± 49	1,500 ± 54	330 ± 50	anaerobic	this study
LmFH-2-H334A	fumarate	4.2 ± 0.2	177 ± 4	196 ± 4	47 ± 3	anaerobic	this study
LmFH-2-R421A	fumarate	4.9 ± 0.7	0.65 ± 0.03	0.72 ± 0.03	0.15 ± 0.03	anaerobic	this study
LmFH-2-D135A	fumarate	1.8 ± 0.3	0.21 ± 0.01	0.23 ± 0.01	0.13 ± 0.03	anaerobic	this study
LmFH-2-T467A	fumarate	3.1 ± 0.5	0.18 ± 0.01	0.20 ± 0.01	0.06 ± 0.01	anaerobic	this study
LmFH-2-R471A	fumarate	2.3 ± 0.7	0.11 ± 0.01	0.12 ± 0.01	0.05 ± 0.02	anaerobic	this study
LmFH-2-R173A	fumarate	2.6 ± 0.6	0.14 ± 0.01	0.15 ± 0.01	0.06 ± 0.02	anaerobic	this study