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. 2020 Mar 11;15(3):e0229445. doi: 10.1371/journal.pone.0229445

Fig 1. R-Spondin 3 is highly expressed in mouse fibrotic livers.

Fig 1

A, Representative fluorescent immunohistochemistry of RSPO1, RSPO2, RSPO3 and α-SMA expression in the livers of mice treated with CCl4 or vehicle control. B, RSPO3 expression pattern in the livers of mice treated with CCl4. Hepatic stellate cells (black arrow), inflammatory cells (red arrow), damaged hepatocytes (black arrowhead). Co-immunofluorescent staining of RSPO3 and CK18 was shown in damaged hepatocytes (white arrow). C, Expression of RSPO3 on CD45+ or F4/80 cells in CCl4 mouse livers. D, β-catenin expression pattern in the livers of mice treated with CCl4. β-catenin nuclear translocation (black arrow). E, RT-PCR of RSPO1, RSPO2 and RSPO3. Values are presented as means ± Stdev. n = 8–10 mice per group. Statistical analysis was conducted using unpaired t- test. ****P <0.0001.