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. 2020 Feb 28;16(2):e1008151. doi: 10.1371/journal.ppat.1008151

Fig 3. IFNα but not other type I or III IFNs induce expression of HIV.

Fig 3

A: Experimental design to determine the effect of IFN on reversal of latent HIV. Resting CD4+ T cells were co-cultured with mDC, infected with EGFP-HIV and day 5 post-infection sorted for CD3+HLA-DR-eFluorhiEGFP-. Sorted cells were cultured in the presence of an HIV integrase inhibitor (RAL) and either left untreated, cultured with 100 U/mL IFN, activated with anti-CD3/CD28+IL-7+IL-2 or activated with anti-CD3/CD28+IL-7+IL-2 in the presence of 100 U/mL IFN B: After 3 days, the cells were harvested and EGP expression quantified by flow cytometry. To calculate the IFN-induced EGFP expression, EGFP expression in unstimulated conditions (background EGFP) was subtracted from EGFP in stimulated conditions. The IFN-induced EGFP expression is then shown as a percentage of maximal stimulation i.e. following stimulation with anti-CD3/CD28+IL-7+IL-2 minus background EGFP expression. Black lines indicate median values and each circle represents an individual donor (n = 7–12 donors). Statistical comparisons were made between IFN treated and untreated samples (top panel) or IFN+anti-CD3/CD28+IL-7+IL-2 and anti-CD3/CD28+IL-7+IL-2 (bottom panel). *p<0.05, ***p<0.001, ns = not significant as determined with Wilcoxon matched pairs signed rank test.