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. 2020 Mar 11;15(3):e0230282. doi: 10.1371/journal.pone.0230282

Fig 5. Schematic representation the proposed vector.

Fig 5

A schematic of the vector developed in this study is shown as pMX, where X = inducible promoter system (A, acetamide; H, hsp60; T, tetRO). The plasmid retains one of the promoters (acetamidase, hsp60, or tetRO) depicted in yellow. Downstream to the promoter is the EcoRV and HpaI restriction enzyme sites that are to be used for cloning purpose. These sites sandwich one of the three tags (viz. 6xHis, GFP, or GST) shown in black. oriM, oriE, and KanR represent the origin of replication for mycobacteria and E. coli, and the kanamycin resistance marker, respectively.