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. 2020 Mar 11;15(3):e0230282. doi: 10.1371/journal.pone.0230282

Table 1. List of oligos used in this study.

Restriction enzyme sites, wherever present, are shown in bold letters.

S. No. Oligo name Sequence (5’ - 3’)
1. pMV_His_For CACCACCACCACCACCACAGTGGTAGTGTTAACTAGCGTACGATCGACTGCCAG
2. pMV_His_Rev GTGGTGGTGGTGGTGGTGACTACCACTGATATCCATATGTGGACTCCCTTTCTCTTATCGGG
3. pMV_NdeI_For GTGCTGTGGTTCATGCTGTTCCTG
4. pMV_SpeI_Rev GTGGTGGCCTAACTACGGCTACAC
5. pMV_GFP_For GATGAGTCCACATATGGATATCAGTGGTAGTATGAGCAAGGGCGAGGAGCTGTTC
6. pMV_GFP_Rev GATGTACGTCAGTTAACACTACCACTCTTGTACAGCTCGTCCATGCCGAG
7. pMV_GST_For GATGAGTCCACATATGGATATCAGTGGTAGTATGTCCCCTATACTAGGTTATTGGAAAATTAAGGG
8. pMV_GST_Rev GATGTACGTCAGTTAACACTACCACTATCCGATTTTGGAGGATGGTCGCCAC
9. B-gal_for ATGCACTGCAGTGGGGATCCC
10. B-gal_rev TTTTTGACACCAGACCAACTGGTAATGG
11. pMV_Tet GGATCCGGAGGAAATCATATGAAATTTGG
12. pMVactseq CCGCAGTTGTTCTCGCATAC
13. pMVfor GCCAGCGTAAGTAGCGG
14. pMV_Tet_Seq GTGACTGCTCGCTACTCTCATCTG
15. pMVrev2 TTGATGCCTGGCAGTCGATC
16. Bgal_NdeI_for CGACGGTTTCCACATGGGGATTGGTGG
17. pMV_NheI_rev CCGTTGAATATGGCTCATAACACCC