Table 2. List of plasmids used in this study.
All the plasmids carry kanamycin resistance marker. pMA, pMT, and pMH represent plasmids carrying acetamidase, tetRO, and hsp60 promoter, respectively. 6xHis, hexa-histidine tag; GFP, Green Fluorescent Protein; GST, Glutathione S-transferases tag.
| S. No. | Name | Backbone | promoter | Reporter tag | Source |
|---|---|---|---|---|---|
| 1. | pMVAcet | pMV261 | acetamidase | None | Lab material |
| 2. | pMEND-Lx | pMINDLx | tetRO | LuxAB | Addgene |
| 3. | pMVTet | pMV261 | tetRO | None | This Study |
| 4. | *pMH-His | pMV261 | hsp60 | 6xHis | This Study |
| 5. | pMH-GFP | pMV261 | hsp60 | GFP | This Study |
| 6. | pMH-GST | pMV261 | hsp60 | GST | This Study |
| 7. | *pMA-His | pMVAcet | acetamidase | 6xHis | This Study |
| 8. | pMA-GFP | pMVAcet | acetamidase | GFP | This Study |
| 9. | pMA-GST | pMVAcet | acetamidase | GST | This Study |
| 10 | pMT-His | pMVTet | tetRO | 6xHis | This Study |
| 11. | pMT-GFP | pMVTet | tetRO | GFP | This Study |
| 12. | pMT-GST | pMVTet | tetRO | GST | This Study |
| 13. | pMA-βgal-His | pMVAcet | acetamidase | 6xHis | This Study |
| 14. | pMT-βgal-His | pMVTet | tetRO | 6xHis | This Study |
| 15. | pMH-βgal-His | pMV261 | hsp60 | 6xHis | This Study |
| 16. | pSD5b | pSD5b | NA | lacZ | Lab material |
| 17. | pMS-QS-NOHGFP | pET21b | T7 promoter | GFP | Lab material |
*pMH-His and pMA-His were earlier named as pSS1 and pSS4, respectively [17].