Table 1. Treatment of neurons with small molecule inhibitors.
Small Molecule | Source, Catalog Number |
Final Concentration | Duration of Pre-treatment |
---|---|---|---|
Ciliobrevin A | Sigma-Aldrich, 250401 | 50 μM | 1 h |
Cycloheximide | Sigma-Aldrich, C7698 | 50 μg/ml | 1 h |
Dynasore | Abcam, ab120192 | 150 μM | 1 h |
E64 | Sigma-Aldrich, E3132 | 200 μM | 4 h |
5-(N-Ethyl-N-isopropyl)amiloride or EIPA | Sigma-Aldrich, A3085 | 25 μM | 1 h |
Genistein | Sigma-Aldrich, G6649 | 200 μM | 1 h |
Jasplakinolide | Sigma-Aldrich, J4580 | 0.5 μM | 20 min |
Nocodazole | Sigma-Aldrich, M1404 | 30 μM | 1 h |
PitStop2 | Abcam, ab120687 | 25 μM | 30 min |
Wortmannin | Cayman Chemicals, 10010591 | 200 nM | 1 h |
List of small molecule inhibitors used in this study along with their sources, final concentrations of use, and durations of pre-treatment of cells.