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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Ann Surg. 2020 Jun;271(6):1174–1185. doi: 10.1097/SLA.0000000000003053

FIGURE 6.

FIGURE 6.

MMP-2 is a direct target of miR-143 and regulates fibroblast collagen levels. A, miR-143 predicted to target MMP-2 3’UTR based on RNA Hybrid algorithm. MMP-2 transcript is NM_004530. Binding position of miR-143 (green) corresponds to position 604–602 of 3′-UTR of MMP-2 (red). B, Luciferase activity in human fibroblasts after transfection with mimic miR-143 and mimic control. Data are mean ± SEM (n = 6) *P < 0.05 compared with mimic control. C and D, Expression of miR-143 in human fibroblasts transfected with (C) miR-143 inhibitor (D) miR-143 mimic. E and F, Expression of MMP-2 in human fibroblasts transfected with (E) miR-143 mimic (F) miR-143 inhibitor. G, Fluorescence microscopy images of collagen 1 (green) Col1 expression in hFb following bolstering of miR143 with mimic miR-143 (upper panel) and knockdown of MMP-2 with siMMP2. Counterstaining was performed using DAPI (blue, nuclear). H, Bar graph presents quantitation of Col1 from (G, upper panel). Data are mean ± SEM (n = 3). *P < 0.05 compared with mimic control. I–J, Knockdown of MMP-2 with siMMP2. Data are mean ± SEM (n = 4). *P < 0.05 compared with si control.