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. 2020 Mar 11;6(11):eaay6093. doi: 10.1126/sciadv.aay6093

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Fig. 5 — (A to C) 3D STED microscopy of an actomyosin aster showing average intensity projection and XZ/YZ intensity profile of actin (A), myosin (B), and the merge (C) demarcating the core and the periphery used for subsequent measurements. Scale bars, 2 μm (XY) and 1.5 μm (XZ/YZ). (D) Intensity plot showing the relative intensities (I/I_max) of actin and myosin along the z axis in the core (solid line) and periphery (dashed lines). Actin intensity peak in the periphery was arbitrarily chosen as position 0. (E) Distance between actin and myosin intensity peaks [as shown in (D)] in the core and the periphery, quantified across seven different asters.