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. 2020 Mar 11;6(11):eaax7515. doi: 10.1126/sciadv.aax7515

Fig. 1. Binding of LukAB to CD11b I-domain of different species.

Fig. 1

(A) Phylogenetic tree based on amino acid sequences of CD11b I-domains from different species and percent amino acid identities to the human I-domain. (B) Coomassie staining of 1 μg of LukAB and I-domains. (C) Binding of LukAB to recombinant I-domains from the species examined in (A) as measured. Data are normalized to the maximum 450-nm absorbance of LukAB bound to the human I-domain. Data are represented as the average of three independent experiments ± SEM. Statistical significance was determined by two-way ANOVA (****P < 0.0001; **P < 0.01; *P < 0.05; ns, not significant) compared to murine I-domain. (D) Linear regression analysis of % binding (relative to human I-domain) at 1 μg/ml and percent amino acid identity to human I-domain.