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. 2020 Mar 11;6(11):eaaz6162. doi: 10.1126/sciadv.aaz6162

Fig. 1. Characterization of 393P and 344SQ cell phenotype and EV protein expression.

Fig. 1

(A) Representative images and (B) graph of the migration and invasion phenotypes of the murine 393P (nonmetastatic) and 344SQ (metastatic) NSCLC cell lines. Scale bar, 100 μm. Data are means ± SEM from three independent experiments; ns, not significant; **P < 0.01. (C) Western blot of EV markers TSG101, HSP70, and CD9 and the Golgi (cytosol) marker GM130 in EVs or whole-cell lysates (WCLs) of 393P and 344SQ cells. (D) Coomassie-stained SDS-PAGE of 393P or 344SQ cell EV isolates; IntDen, relative mean and SD of the integrated lane densitometry from three replicates. N/A, not applicable. (E) Venn diagram of EV proteins identified by LC-MS/MS. (F) Western blot of proteins in EVs, WCLs, and EV-depleted medium. BP, binding protein. (G) Heat map of 393P versus 344SQ EV Western blot expression from low (light red) to high (dark red) optical density.