Fig. 5. Survival is mediated via adhesion-FAK-ERK signaling.

(A) Experimental timeline of inhibitor dosing. Day 7 experiments were dosed continually through a day 7 endpoint; day 28 experiments were dosed continually through a day 28 endpoint; separate cultures were established for 21 days, where inhibitor dosing was initiated for an additional 7 days (dose-established culture). (B) HCC 1954 survival at day 7 on collagen coverslips with selected inhibitors dosed for the duration of the experiment. (C) Survival of HCC 1954 cells after establishment of dormant culture for 21 days and then subsequent dosing with inhibitors through days 21 to 28. (D) HCC 1954 survival at day 7 with inhibitors when seeded onto HCC 1954 decellularized ECM. Black, control; blue, anti-β₁ (MAB17781; 1 μg/ml); green, FAK inhibitor 14 (10 μM); purple, PD0325901 (MEK inhibitor, 10 μM); red, FR180204 (ERK inhibitor, 20 μM); gray, cilengitide (100 μM). P < 0.05 is denoted with *, P ≤ 0.001 with *** and P ≤ 0.0001 with ****.