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. 2020 Mar 3;9:e52570. doi: 10.7554/eLife.52570

Figure 1. p16 expression in NP cells from human interverbal discs with different degrees of degeneration (G2–G5 groups according to Pfirrmann grade).

(A) Representative images of H and E staining showing cell/tissue general morphology. Safranin O staining with collagen and NP cells appearing orange and fibers blue/violet. Masson staining with collagen and NP cells appearing blue and fibers red; and immunohistochemical staining for p16. (B) Quantification of p16-positive cells (%). p16 protein levels were assessed by (C) western blotting and (D) measured by densitometric analyses and expressed as folds relative to grade 2 (G2) NP samples. Data are presented as mean ± SD (n = 3); *p<0.05; **p<0.01.

Figure 1—source data 1. Source data and related summary statistics for Figure 1A and C.
elife-52570-fig1-data1.xlsx (189.2KB, xlsx)

Figure 1.

Figure 1—figure supplement 1. Representative magnetic resonance imaging (MRI) scans of patients with different categories of disc degeneration according to Pfirrmann grade.

Figure 1—figure supplement 1.

Grades 2–5: G2–5. The yellow arrow indicates the surgical site.
Figure 1—figure supplement 2. Pffirmann grade of the degenerated disc from the human specimens correlates individually with p16 expression. ***p<0.001.

Figure 1—figure supplement 2.

Figure 1—figure supplement 2—source data 1. Related summary statistics.